In the toxicity testing of electronic cigarettes, multiple foreign tobacco companies have adopted different toxicological and chemical analysis methods to compare the safety of traditional cigarettes and electronic cigarette products, in order to demonstrate the low risk advantage of electronic cigarette products.

Electronic cigarette oil testing project

Propylene glycol, glycerol, nicotine, diethylene glycol, nitrosamine, diacetyl (2,3-butanedione), phthalate, heavy metals, etc


Quality Testing Standards for Electronic Cigarettes

SN/T Import and Export Electronic Fuming Liquid Inspection Regulations

T/CECC-1-2017 General Specification for Electronic Fuming Appliances Products

T/CECC 2-2017 Specification for Electronic Fuming Fluids

YC/T559-2018 Determination of alkaloids as characteristic components of tobacco by gas chromatography-mass spectrometry and gas chromatography-tandem mass spectrometry

YC/T560-2018 Determination of hydrogen stable isotope ratio in nicotine, a characteristic component of tobacco. Gas chromatography-stable isotope ratio mass spectrometry method

YC/T561-2018 Determination of the ratio of nicotine optical isomers as characteristic components of tobacco - High performance liquid chromatography and ultra high performance liquid chromatography tandem mass spectrometry

Through relevant research on animal experiments, it has been found that the inhalation of electronic cigarettes in test animals mainly causes changes in the tissues and cells of their respiratory system. It can be seen that the respiratory system is the most direct target organ of electronic smoke. Choosing respiratory cells as evaluation cells is conducive to evaluating the short-term biological effects of electronic smoke on the body.


The in vitro toxicological testing of electronic cigarettes specifically includes

Collection of mainstream flue gas samples.

Preparation and collection of mainstream tobacco smoke for in vitro toxicological assessment.

Neutral red test: Evaluate the toxicity of the test substance using the half lethal rate of cells.

Neutral Red Uptake Assay. Assess cytotoxicity of test sample by half maximal inhibitory concentration (IC50).

Bacterial reverse mutation test: Use the mutagenicity of bacteria to evaluate the toxicity of the test substance.

Bacterial Reverse Mutation Assay (Ames Assay). Assess cytotoxicity of test sample by bacterial mutagenicity.

In vitro micronucleus test: A report evaluating the toxicity of the test substance using the nuclear micronucleus rate.

In Vitro Micronucleus Assay. Assess cytotoxicity of test sample by ratio of micronucleated cells per 1000 observed cells.